Spectrofluorimetric analysis in vivo : applications to kinetics of ALA induced fluorescence
نویسندگان
چکیده
منابع مشابه
بررسی مقایسهای دوزیمتری PpIX تولید شده درون تومور پس از مصرف ALA در درمان فوتوداینامیک به دو روش فلوئوریمتری و فلوئورسانس اسپکتروسکوپی
Background: The important issue in the application of Photodynamic Therapy (PDT), as one of the fundamental basis of new treatment for cancerous lesions, is optimal dosimetry. In this context, it is necessary to obtain enough information about the drug concentration within the target tissue. On the other hand, the fact that ALA itself is not a photosensitizer and causes the production of Pr...
متن کاملA Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy
Introduction During the process photodynamic therapy (PDT), bleaching of photosensitizer induced by irradiation and generation of reactive oxygen species (ROS) can provide some information concerning the efficiency of treatment. Since gold nanoparticles (GNPs) have been highlighted as efficient drug delivery systems, in this study, by utilizing GNPs conjugated with 5 aminolevulinic acid (5-ALA-...
متن کاملCoupling Second-Order Excitation-Emission Spectrofluorimetric Data with Standard Addition method to Quantify Carvedilol in Real Samples
Prediction using pure standards is expected to be biased whenever the slope of the calibration is affected by the presence of sample matrix. Moreover, in the presence of unknown spectral interferents, first-order algorithms like partial least squares cannot be used. In this study, a method for determination of carvedilol (CAR) in tablet and urine samples is proposed by excitation-emission fluor...
متن کاملKinetics and subcellular localization of 5-ALA-induced PpIX in DHL cells via two-photon excitation fluorescence microscopy.
Two-photon excitation fluorescence (TPEF) microscopy was used to measure the 5-aminolevulinic acid (5-ALA)-induced PpIX fluorescence in follicular lymphoma DHL cells. Kinetics of 5-ALA-induced PpIX accumulation in DHL cells under various 5-ALA concentrations was studied. We found that during the course of continuous incubation with 5-ALA, the relationship between the DHL cell fluorescence signa...
متن کاملFluorescence spectra of cardiac myosin and in vivo experiment: studies on daunorubicin-induced cardiotoxicity
Objective(s):The objective of this study was to investigate the interaction of daunorubicin (DNR) and cardiac myosin (CM) and the changes in mice hearts to exhibit DNR-induced cardiotoxicity . Materials and Methods:The interaction between DNR and CM was expressed using fluorescence quenching at pH 4.0-9.0 and 15-37 °C. DNR-induced cardiotoxicity was studied using in vivo experiment. Forty grou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016